During our 30 years of restriction enzyme research, we compiled one of the largest collections of restriction enzyme producing bacterial strains in the industry. Thermo Scientific™ FastDigest™ restriction enzymes Prepare the reaction mixture at room temperature in the order indicated: Component Volume Plasmid DNA PCR product** Genomic DNA Water,* nuclease-free (Cat. Restriction Enzyme Digestion and Ligation | Thermo Fisher Scientific - DE Restriction Enzyme Digestion and DNA Modification Cloning by restriction enzyme digestion and ligation is a simple and easy way of moving a fragment of double-stranded DNA from one plasmid to another. Search Thermo Fisher Scientific. Simplicity of FastDigest restriction enzymes, a universal system using one buffer vs. the complicated NEB ™ offering. Peak DNA digestion without star activity is best accomplished with conventional Thermo Scientific restriction enzymes using the Five Buffer System. Fisher Scientific SAS - Boulevard Sébastien Brant - F67403 Illkirch Cedex - France Tél. Incubation was done at 37°C for 15 minutes. • Double and multiple digestions in a universal buffer for any combination of enzymes • No sequential digestions and buffer changes • 176 unique specificities • Complete digestion in 5–15 minutes • Direct loading of reaction mixture on gels Figure 1. 03 88 67 11 68 - fr.fisher@thermofisher.com S.A.S. Thermo Scientific™ FastDigest SalI Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 03 88 67 14 14 - Fax.

Thermo Scientific FastDigest enzymes are an advanced line of restriction enzymes for rapid DNA digestion. It’s that easy. Restriction Enzymes–Thermo Scientific FastDigest Restriktionsenzyme – Thermo Scienti 100% activity in a single buffer The FastDigest Green Buffer and Thermo Scientific FastDigest Buffer are proprietary digestion buffers which support 100% activity of all FastDigest restriction enzymes. Na - Oz Restriction Enzymes Thermo Scientific™ GTP Solution (100 mM) Optimize molecular biology applications with this GTP, a highly stable nucleotide with 99% purity.

Marque: Thermo Scientific™ ER0271 34.95 EUR valable jusqu'au 2020-06-30 Thermo Scientific™ FastDigest™ XbaI Effectuez une digestion de l’ADN simple, double ou multiple en 5 à 15 minutes à peine grâce à cette gamme avancée d’enzymes de restriction actives à 100 % dans un seul tampon universel. The Invitrogen Anza Restriction Enzyme Cloning System is a complete, one-buffer system of restriction enzymes and DNA-modifying enzymes.
Thermo Scientific FastDigest enzymes are an advanced line of restriction enzymes for rapid DNA digestion. Reaction mixtures included 1 µg of DNA and 1 µL of each restriction enzyme to a total volume of 30 µL for triple digestion, per the recommended protocol.
Search All. Restriction Enzymes–Thermo Scientific ‹ Thermo Scientific Restriction & Modifying Enzymes. During our 30 years of restriction enzyme research, we compiled one of the largest collections of restriction enzyme producing bacterial strains in the industry. This system allows for double and multiple digestions with any combination of enzymes. Request a sample.